Coding

Part:BBa_K3788024:Design

Designed by: Rebecca Pagès   Group: iGEM21_Aix-Marseille   (2021-09-29)


6His_conA_linker_conA


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 165
    Illegal BglII site found at 1141
    Illegal XhoI site found at 102
    Illegal XhoI site found at 1078
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 427
    Illegal AgeI site found at 1403
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 917
    Illegal SapI site found at 929


Design Notes

After the sequence eventual mutation was prevented : Amino acids have been changed to avoid the formation of a stop codon and also to remove restriction sites corresponding to RFC10 and RFC25.



Source

The ConA sequence was taken from uniprot sequence of ConA and liker was created by ourself


References